Protein verification with Aptamers
Patent 14035/TUB

This Innovation enables the easy marking and separating of different Proteins in a single sample.

  1. Certification of multiple proteins in one sample
  2. Feasibly in vitro
  3. High specificity
  4. Cheaper and faster than state of the art
  5. No denaturation of the protein needed
Possible Applications

Diagnostics, biological and biochemical analytics


The main methods of detection for proteins are methods like ELISA or Western/Dot Blot. They are based on antibodies and thereby are dependent on animal experiments, not always creating exactly identical anti bodies. The use of aptamers presented itself as a good alternative. But the missing specificity especially for a protein mixture is a problem for broader applications.

Technical Description

The innovation presented here is based on a technique for separating different proteins inside a sample and subsequent in vitro marking with aptamers. This enables the specific verification of the protein. The separation takes place by native separation techniques, keeping the complex folding of the protein intact and utilizing different parameters, particularly the isoelectric point within a gel electrophoresis. The aptamer binds highly specific to the protein to be verified and can be marked for easy certification. Here all common markings are possible. Due to the separation of the proteins, the verification of various proteins in a single sample is possible with the technique presented here. Here the absence of the protein to be certified also counts as evidence.

Contact Us

Ina Krüger

Technology Transfer Manager

+49 (0)30 314-75916

Technology Readiness Level

Technology validated in lab

Property Rights

pending: US
approved: CH, DE, EP, FR, GB

Patent Holder

Technische Universität Berlin

Possible Cooperation
  • R&D Cooperation
  • Licensing
  • Patent Purchase